Translational_Unit

Part:BBa_K1500014

Designed by: Ryan Beckner   Group: iGEM14_UChicago   (2014-10-17)

paroF->MCherry-MutH(E56A)

This biobrick contains mutH(E56A) and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH(E56A) and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached (florescence will decrease). mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1346
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None